Information

Is there a stem cell dye for education purposes?

Is there a stem cell dye for education purposes?


We are searching data for your request:

Forums and discussions:
Manuals and reference books:
Data from registers:
Wait the end of the search in all databases.
Upon completion, a link will appear to access the found materials.

I need a dye that will stain stem cells (for this purpose the stem cells will proquired from chicken bone marrow).

This will be an educational hands on activity for young teenagers so ideally the dye should be:

  • Safe
  • Easy to handle
  • Stain quickly
  • Be relatively cheap
  • Present an obvious colour change under a low zoom microscope or the naked eye

I vaguely remember using a dye years ago that fits this bill, but cannot recall the name or company.


There is no specific dye for stem cells. You would have to do an immuno-histochemical staining for stem cell markers such as Sox2/Oct4 etc.

Usually stem cells have a distinct morphology (round and clustered). You can use Leishman's (or Romanowsky-Giemsa) stain.


How about Brdu… ? Normally, stem cells have active proliferation, their dividing are fast, thus they could uptake Brdu, and this is one of basic stem cell test in papers. I think it isn't too expensive, comparing with antibodies… but you may need UV-light, and don't let children play Brdu. It is carcinogen, however, I think it only a very high dose can cause a cancer…


What Does an Iodine Stain Do in Biology?

If you’re in the biology lab, you must choose your substances carefully for safety purposes and to obtain correct results. Under the microscope, you’ll find that some slide specimens are nearly transparent without the help of some stain, but you don’t want to throw just anything on the slide to help you out. Iodine is one of the many lab-safe stains that will assist you in making better observations.


Going Home

Even though you may be very happy and feel ready to go home, you might also feel nervous. It’s normal to have some worries and concerns as your discharge date gets closer. At home, you and your family will need to manage your care. Feeling confident and comfortable doing this takes time. During your follow-up visits, your social worker can help you get the services you need and give you emotional support.

When you go home, it will take time to get used to living at home again. You may find that the things you need to do to keep from getting sick add some stress to your life. Feeling a sense of balance and comfort again will come with time. Try to stay as calm and confident as you can.

Your recovery after transplant will be gradual. You probably won’t feel the same way you did before your illness for a while. You may feel tired and weak, have a smaller appetite, and notice changes in the way things taste and smell. It will also take time for you to get your strength back and go back to doing the activities you enjoyed before your illness and transplant.


Cell Proliferation and Cytotoxicity Assays

Cell viability is defined as the number of healthy cells in a sample and proliferation of cells is a vital indicator for understanding the mechanisms in action of certain genes, proteins and pathways involved cell survival or death after exposing to toxic agents. Generally, methods used to determine viability are also common for the detection of cell proliferation. Cell cytotoxicity and proliferation assays are generally used for drug screening to detect whether the test molecules have effects on cell proliferation or display direct cytotoxic effects. Regardless of the type of cell-based assay being used, it is important to know how many viable cells are remaining at the end of the experiment. There are a variety of assay methods based on various cell functions such as enzyme activity, cell membrane permeability, cell adherence, ATP production, co-enzyme production, and nucleotide uptake activity. These methods could be basically classified into different categories: (I) dye exclusion methods such as trypan blue dye exclusion assay, (II) methods based on metabolic activity, (III) ATP assay, (IV) sulforhodamine B assay, (V) protease viability marker assay, (VI) clonogenic cell survival assay, (VII) DNA synthesis cell proliferation assays and (V) raman micro-spectroscopy. In order to choose the optimal viability assay, the cell type, applied culture conditions, and the specific questions being asked should be considered in detail. This particular review aims to provide an overview of common cell proliferation and cytotoxicity assays together with their own advantages and disadvantages, their methodologies, comparisons and intended purposes.


Why Is Iodine Stain Used on Onion Cells?

Iodine is often used to stain onion cells before microscopic examination to enhance the visibility of the cells. Many cells, including those of onions and other vegetables, are often transparent. When unstained cells are viewed under a microscope, the light passes directly through the cells&rsquo various structures revealing little to no detail. By contrast, when iodine or other dyes are used, the cell absorbs the dye into its various organelles and structures, which blocks the light and allows the observer&rsquos eye to detect the details of the cell.

Iodine is not the only dye used in microscopic examinations. Iodine, food coloring, malachite green and methylene blue are common and easily accessed dyes. Malachite green and methylene blue are both available at speciality aquarium stores, but iodine and food coloring are available at most drug stores and grocery stores.

Different types of cell structures work better with different dyes. Because of this, scientists can use a dye that is tailored to the specific organelle. Additionally, scientists can use multiple dyes that highlight many different parts of a plant or animal&rsquos cells. Scientists must always exercise care because dyes not only stain cells. They may also stain the hands and skin of the scientists.


Common Stains for Slide Preparation

Since most biological structures are transparent, we need a technique to distinguish clearly the parts as we observe under the microscope. This is possible by employing some means by which contrast between different structures can be obtained. Sometimes adjusting light helps but the most common method is staining.

Stains such as methylene blue in low concentrations does not harm the tissues and so can be safely used on living materials. Such stains are called vital stains.

For making temporary slides stains such as methylene blue, idodine, aniline hydrochloride, safranin etc are used.

Given below are some common stains and their uses and the colour they show up as:

Iodine: Stains carbohydrates in plant and animal specimens brown or blue- black.Stains glycogen red.

Methylene blue: Stains acidic cell parts (like nucleus) blue. Use on animal, bacteria and blood specimens. Can be used as a substitute for Janis B green.

Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. Use on plants,animals and blood. Can be used as a substitute for Congo Red and Carmine.

Safranin : Mainly used for sections of plant tissues, stains red

Toluidene blue: Stains acidic cell parts (like nucleus) dark blue. Good to show mitosis in plant cells.

Wright’s stain: Stains red blood cells pink/red.

Leishman’s stain: Stains nucleus of WBC blue and blood cells pink

Crystal Violet: Stains bacteria purple

Aceto-orcein: Biological stain for chromosomes and connective tissue.

Sudan III: Biological stain used as a lipid indicator.

1 comment to Common Stains for Slide Preparation

can you give memore information about slides stains and detail yours thanks leon


A Review of the Types of Chemical Bonds

Ionic Bonds - An ionic bond is a complete transfer of electrons from one atom to another. This generally happens between atoms that have opposite electronegativity. This means one has very few atoms in their outer shell, while the other has many. A common example of an ionic bond is that of salt, with Na and Cl. Sodium has one electron in its outer shell, in which it transfers to chloride to make an ionic bond.

Covalent Bonds - Covalent bonds involve a complete sharing of electrons between two atoms. It occurs most commonly between atoms that have outer shells that are only partially filled. If the two atoms have similar electronegativity, then the electrons can be shared between them. Carbon forms covalent bonds.

Polar Covalent Bonds - A polar covalent bond is much like a covalent bond, except that it occurs between atoms that have differing electronegativity. When this happens, the electrons are still shared, but they tend to spend more time around the more electronegative atom versus the other. Such an example is with water. Oxygen is very electronegative, while hydrogen is not. The electrons tend to favor oxygen and spend more time around it.

Hydrogen Bonds - Hydrogen bonds are less of chemical bonds and are more of a static attraction. They involve the reaction between a hydrogen atom and an electronegative atom. The strength of a hydrogen bond is less than a tenth of a covalent bond.


Stem Cell Research

Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies.

Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, genomics and translational research. Special focus of SCR is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines.

&bull Original articles
&bull Short reports
&bull Review articles
&bull Communications
&bull Methods and reagents articles
&bull Lab Resource: Stem Cell Line


MICROBIOLOGY

This Blog is extremely on Microbiology and its related fields.Microbiology often has been defined as the study of organisms and agents too small to be seen clearly by the unaided eye—that is, the study of microorganisms. Because objects less than about one millimetre in diameter cannot be seen clearly and must be examined with a microscope.

Stains/ Dyes

  • Get link
  • Facebook
  • Twitter
  • Pinterest
  • Email
  • Other Apps

STAINS/ DYES
They are colored organic compounds used for staining microorganisms. Chemically,
Stains= Benzene ring+ Chromophore+ Auxochrome

According to nature of stain, it can be classified into:

1. Acidic Dyes: It is dye which has negative charge so they bind to positively charged cell structures like some proteins. Acidic dyes are not very often used in Microbiology lab.except to provide background staining like Capsule staining. Examples: Nigrosine, Picric acid, Eosin, Acid fuschin, India ink etc.

2. Basic Dyes: This dye have positive charge & bind to negatively charged molecules(nucleic acid, -COOH -OH). Since, surface of bacterial cells are negatively charged(due to Teichoic acid), basic dyes are most commonly used in bacteriology. Examples: Crystal Violet, Methylene Blue, Safranin , basic fuschin.

3. Neutral Dyes: They are usually formed from precipitation in which are produced when aqueous acidic & basic stains are combined. Neutral dyes stains nucleic acids, & cytoplasm. Eg Eosinate of Methylene blue, Giesma stain.


Stem cell transplants and fertility

Most people who have stem cell transplants become infertile (unable to have children). This is not caused by the cells that are transplanted, but rather by the high doses of chemo and/or radiation therapy used. These treatments affect both normal and abnormal cells, and often damage reproductive organs.

If having children is important to you, or if you think it might be important in the future, talk to your doctor about ways to protect your fertility before treatment. Your doctor may be able to tell you if a particular treatment will be likely to cause infertility.

After chemo or radiation, some women may find their menstrual periods become irregular or stop completely. This doesn’t always mean they cannot get pregnant, so birth control should be used before and after a transplant. The drugs used in transplants can harm a growing fetus.

The drugs used during transplant can also damage sperm, so men should use birth control to avoid starting a pregnancy during and for some time after the transplant process. Transplants may cause temporary or permanent infertility for men as well. Fertility returns in some men, but the timing is unpredictable. Men might consider storing their sperm before having a transplant.

For more information on having children after being treated for cancer or sexual problems related to cancer treatment, see Fertility and Sexual Side Effects.